Circadian regulation of metabolic, cell division, and cation transport promoters in the gastrointestinal bacterium Klebsiella aerogenes

Introduction All eukaryotes and at least some prokaryotes express the capacity to anticipate adapt daily changes of light temperature in their environments. These circadian programs are fundamental features many forms life. Cyanobacteria were first have demonstrated gene expression. Recently, a rhythm was also discovered an unrelated bacterium, Klebsiella aerogenes , human gut commensal nosocomial pathogen. Methods Here we characterize new clock-controlled genes with spatial differences expression using bacterial luciferase reporter. include dephospho-coenzyme A kinase ( coaE ), manganese transporter, H-dependent mntH ) identified as filamenting temperature-sensitive mutant Z ftsZ ). Results Discussion The data show that all three reporter constructs exhibited variation, although only PmntH::luxCDABE strains synchronized by melatonin. Additionally, K. divides rhythmically vitro these bacteria may alternate between exponential stationary cells. Together, findings provide deeper understanding .